Blood parameters, supplies, and number of personal required, along with stress of the animal were evaluated using 2 different jugular vein puncture methods. The jugular vein is one of a few collection sites which can be used for multiple blood collections in a conscious rat. Blood sampling can be stressful for animals because of the handling, restraint, anaesthesia, or discomfort associated with a particular technique. The removal of blood at multiple time points from an animal is required for these evaluations. Pharmakokinetics and toxicokinetics are important components of drug discovery and development process. P2 A Unique Blood Collection Method in a Conscious RatĪM Walters *1, M Hirashima 1, K Tsuyama 1, S Wakamatsu 2ġSNBL USA Ltd, Everett, WA 2Shin Nippon Biomedical Laboratories, LTD (SNBL) Japan, Kagoshima, Japan The modified connector further allows for enhancement to the welfare of the animals by providing social enrichment through pair and group housing without compromising the integrity of the surgical model. The end result is a connector that allows for dosing and timed collections of high-quality blood samples using a response movement caging automated blood sampling system. All dosing and blood sample collections were performed in accordance with an IACUC-approved animal use protocol. Studies were conducted using our standard inhouse study design for pharmacokinetic studies with multilple blood sample collections out to 24 hours post dose.
The modified connector allows the standard extension line from the response movement caging system to easily connect for blood sample withdraws. The newly designed custom connector was primed and attached to the port after removal of the protective metal cap. Animals were dual jugular cannulated using the available port/protective metal cap to allow for pair or group housing. A sterile custom connector was designed in collaboration with an industry vendor. To do this, we modified the automated blood sample connection to be compatible with the available port/protective cap system. Our goal was to develop a vessel-cannulated surgical model that could be socially housed and would be compatible with response movement caging.
A port/protective cap model is available that allows for pair and group housing of cannulated animals and is compatible with a harness/swivel automated blood sampling system. Traditional practice has been to single-house vessel-cannulated rodents post surgically to protect exposed exteriorized catheters. P1 A Novel Vascular Button Connection Using Combined Technologies while Allowing for Social Enrichment by Pair HousingĪJ Hehman *1, A Zuvich 1, KA Adams 2, D Shuey 1ġToxicology, Incyte Corporation, Wilmington, DE 2Laboratory Animal Resources, Incyte Corporation, Wilmington, DE
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